The chapel was built by Bishop Girolamo Basso della Rovere, nephew of Pope Sixtus IV, from 1471 to 1484. The architecture is attributed to Andrea Bregno. The painted decoration is attributed to Pinturicchio and his workshop, who worked here between 1484 and 1492.

The small chapel is hexagonal with a sexpartite ribbed vault and the entrance is protected by a slim balustrade. Fake porphyry columns with Corinthian capitals support an entablature of white and gilded marble. They are placed on a pedestal which is decorated with painted benches and illusionistic monochrome reliefs.