The second altar to the right is dedicated to the Immaculate Conception of Our Lady, and has a pair of grey granite Corinthian columns. The altarpiece is by Fiammeri again, and shows Our Lady in Glory with symbols associated with her.

On the pediment frieze of the aedicule is a quotation from Sirach 43:2: "Wonderful instrument, a work of the Most High". Above the aedicule is a depiction of King Solomon.